Stomatin Antibody

• Catalog Number: orb1523019
• Category: Antibodies
• Description: Stomatin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 238 and 288
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Stomatin
• UniProt ID: P27105
• NCBI: NP_004090.4
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: erythrocyte membrane protein band 7.2 (stomatin);
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Stomatin by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN and RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Stomatin antibody orb1523019 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Stomatin by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using RIPA buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-Stomatin antibody orb1523019 used for IP at 6 µg per reaction.

Detection of human Stomatin by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma infiltrating cells. Antibody: Affinity purified rabbit anti-Stomatin (Cat. No. orb1523019) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB