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TNFRSF1A Antibody
Description
Images & Validation
−| Tested Applications | ELISA, NeA, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsTNF Receptor-I-I (human-soluble Tumor Necrosis Factor Receptor-I). |
| Target | TNFRSF1A |
| Purification | Anti-hsTNF Receptor-I specific antibody was purified by affinity chromatography employing immobilized hsTNF Receptor-I matrix. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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To detect hsTNF-receptor by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human sTNF-receptor (orb1272301) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor.

To detect hsTNF-receptor by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hsTNF-receptor by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
TNFRSF1A Antibody (orb1272302)
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