STK33 monoclonal antibody (M02), clone 6G1

• Catalog Number: orb2290843
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant STK33.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 6G1
• Tested applications: ELISA, IF, IP, WB
• Reactivity: Human
• Isotype: IgG1 Kappa
• Immunogen: STK33 (AAH31231, 1 a.a. ~ 514 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MADSGLDKKSTKCPDCSSASQKDVLCVCSSKTRVPPVLVVEMSQTSSIGSAESLISLERKKEKNINRDITSRKDLPSRTSNVERKASQQQWGRGNFTEGKVPHIRIENGAAIEEIYTFGRILGKGSFGIVIEATDKETETKWAIKKVNKEKAGSSAVKLLEREVNILKSVKHEHIIHLEQVFETPKKMYLVMELCEDGELKEILDRKGHFSENETRWIIQSLASAIAYLHNNDIVHRDLKLENIMVKSSLIDDNNEINLNIKVTDFGLAVKKQSRSEAMLQATCGTPIYMAPEVISAHDYSQQCDIWSIGVVMYMLLRGEPPFLASSEGKLFELIRKGELHFENAVWNSISDCAKSVLKQLMKVDPAHRITAKELLDNQWLTGNKLSSVRPTNVLEMMKEWKNNPESVEENTTEEKNKPSTEEKLKSYQPWGNVPETNYTSDEEEEKQSTTYEKQFPATSKDNFDMCSSSFTSSKLLPAEIKGEMEKTPVTPSQGTATKYPAKSGALSRTKKKL
• NCBI: AAH31231
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged STK33 is 1 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to STK33 on HeLa cell. [antibody concentration 25 ug/ml]

Immunoprecipitation of STK33 transfected lysate using anti-STK33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STK33 MaxPab rabbit polyclonal antibody.

STK33 monoclonal antibody (M02), clone 6G1 Western Blot analysis of STK33 expression in HeLa.

Western Blot analysis of STK33 expression in transfected 293T cell line by STK33 monoclonal antibody (M02), clone 6G1. Lane 1: STK33 transfected lysate(57.9 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of STK33 over-expressed 293 cell line, cotransfected with STK33 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with STK33 monoclonal antibody (M02) clone 6G1 (Cat # orb2290843). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (82.28 KDa).