STK3 Antibody

• Catalog Number: orb1241100
• Category: Antibodies
• Description: STK3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human STK3.
• Concentration: batch dependent
• Dilution range: STK3 antibody can be used for detection of STK3 by ELISA at 1:312500. STK3 antibody can be used for detection of STK3 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 56 kDa
• Target: STK3
• UniProt ID: Q13188
• NCBI: NP_006272
• Storage: For short periods of storage (days) store at 4°C. For longer periods of storage, store STK3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: STK3, FLJ90748, KRS1, MST2
• Note: For research use only
• Application notes: STK3 antibody can be used for detection of STK3 by ELISA at 1:312500. STK3 antibody can be used for detection of STK3 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Expiration Date: 12 months from date of receipt.

Antibody used in WB on Human cell lines at: 1:2000 (Lane 1: 30 ug of HeLa cell lysate, Lane 2: 30 ug of 293T cell lysate).

Antibody used in WB on transfected Cos-7 at: 1:2000 (Lane 1: 100 ug untransfected COS-7 lysate, Lane 2: 100 ug mock transfected Cos-7 lysate, Lane 3: 100 ug STK3 transfected Cos-7 lysate, Lane 4: 50 ug STK3 transfected Cos-7 lysate, Lane 5: 25 ug STK3 transfected Cos-7 lysate).

Antibody used in WB on transfected Cos-7 at: 1:2000 (Lane 1: 100 ug untransfected RPE-1 lysate, Lane 2: 100 ug untransfected RPE-1 lysate, Lane 3: 100 ug STK3 induced RPE-1 lysate, Lane 4: 100 ug STK3 induced RPE-1 lysate).

Antibody used in WB on Human Jurkat 0.2-1 ug/ml.