Stefin B CSTB Antibody (monoclonal, 2B6)

• Catalog Number: orb421124
• Category: Antibodies
• Description: Stefin B CSTB Antibody (monoclonal, 2B6)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2B6
• Tested applications: FC, ICC, IHC, IHC-Fr, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: E. coli-derived human Stefin B recombinant protein (Position: M1-F98). Human Stefin B shares 78.6 % amino acid (aa) sequence identity with both mouse and rat Stefin B.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 14 kDa
• UniProt ID: P04080
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cystatin-B; CPI-B; Liver thiol proteinase inhibito
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of PC-3 cells using anti-Stefin B antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Stefin B using anti-Stefin B antibody.Lane 1:human U-87MG cell;2:human SW620 cell;3:human U-937 cell;4:human Caco-2 cell.

IHC analysis of Stefin B using anti-Stefin B antibody.Stefin B was detected in paraffin-embedded section of human tonsil tissue.