Staufen1 Antibody

• Catalog Number: orb1523865
• Category: Antibodies
• Description: Staufen1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 1 and 50
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Staufen1
• UniProt ID: O95793
• NCBI: NP_059347.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: staufen, RNA binding protein, homolog 1; PPP1R150;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Staufen1 by western blot. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-Staufen1 antibody orb1523865 used for IP at 6 µg/mg lysate. Staufen1 was also immunoprecipitated by rabbit anti-Staufen1 antibody. For blotting immunoprecipitated Staufen1, orb1523865 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human and mouse Staufen1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, mouse Renca, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-Staufen1 antibody orb1523865 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.