SRC (phospho-S75) antibody

• Catalog Number: orb214604
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SRC
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S75 of human c-SRC protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: SRC
• Entrez: 6714, 20779, 83805
• UniProt ID: P05480, Q9WUD9, P12931
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti SRC1 antibody, anti Proto-oncogene tyrosine-p
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of c-SRC (Phospho-S75) expression in HeLa (A), HuvEc (B), Jurkat (C), COLO205 (D), HEK293T EGF-treated (E), A431 pervanadate-treated (F) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 60 kD)

Immunohistochemical analysis of c-SRC (Phospho-S75) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.