SPTLC2 Antibody

• Catalog Number: orb1240010
• Category: Antibodies
• Description: SPTLC2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Anti-SPT2 antibody (orb1240010) was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human SPT2. The immunogen is located within the last 50 amino acids of SPT2.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-1 μg/mL; ICC: 10 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 63kDObserved: 63 kD
• Target: SPTLC2
• UniProt ID: O15270
• NCBI: NP_004854
• Storage: SPT2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SPT2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SPT2 Antibody: LCB2, SPT2, HSN1C, LCB2A, NSAN1C, h
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: SPT2, orb1240010 (1 µg/mL), SPT2 (1 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: SPT2, orb1240010 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse 3T3 Cell Lysate in (A) the absence and (B) the presence of blocking peptide. Loading: 15 µg of lysates per lane. Antibodies: SPT2, orb1240010 (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Brain Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: SPT2, orb1240010 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse 3T3/NIH Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: SPT2, orb1240010 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry Validation of SPT2 in Mouse 3T3 Cells. Immunocytochemical analysis of 3T3 cells using anti-SPT2 antibody (orb1240010) at 10 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of SPT2 in Mouse 3T3 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed 3T3 Cells labeling SPT2 with orb1240010 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).