SPTLC1 Antibody

• Catalog Number: orb334627
• Category: Antibodies
• Description: SPTLC1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SPTLC1 (444-473aa IRVVVTVEQTEEELERAASTIKEVAQAVLL), different from the related mouse sequence by three amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 52744 MW
• UniProt ID: O15269
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Serine palmitoyltransferase 1;2.3.1.50;Long chain
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-SPTLC1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of SPTLC1 using anti-SPTLC1 antibody.Lane 1:human placenta tissue; 2:human U-87MG cell; 3:human A431 cell; 4:human PC-3 cell;5:human U20S cell;6:human HepG2 cell;7:human U-937 cell.

IF analysis of SPTLC1 using anti-SPTLC1 antibody.SPTLC1 was detected in immunocytochemical section of U20S cell.

IHC analysis of SPTLC1 using anti-SPTLC1 antibody.SPTLC1 was detected in paraffin-embedded section of human intestinal cancer tissues.