SPTBN2 Antibody

• Catalog Number: orb1252205
• Category: Antibodies
• Description: SPTBN2 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPTBN2/1584
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this Spectrin beta III antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTWB: 0.5-1 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Spectrin beta III antibody to be titered up or down for optimal performance.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SPTBN2
• UniProt ID: O15020
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTWB: 0.5-1 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Spectrin beta III antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human pancreas with Spectrin beta III antibody (clone SPTBN2/1584). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE mouse kidney with Spectrin beta III antibody (clone SPTBN2/1584). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE rat pancreas with Spectrin beta III antibody (clone SPTBN2/1584). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free Spectrin beta III Antibody (clone SPTBN2/1584). Confirmation of Integrity and Purity of the Antibody.