SPTAN1/Alpha II-spectrin Antibody

• Catalog Number: orb1528757
• Category: Antibodies
• Description: SPTAN1/Alpha II-spectrin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse
• Immunogen: between 2422 and 2472
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:50 - 1:250
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SPTAN1/Alpha II-spectrin
• UniProt ID: Q13813
• NCBI: NP_003118.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: spectrin alpha chain, non-erythrocytic 1; EIEE5; S
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse SPTAN1/Alpha II-Spectrin by western blot.Samples: Whole cell lysate from HeLa, HEK293T, and mouse NIH 3T3 cells. Antibody: Affinity purified rabbit anti-SPTAN1/Alpha II-Spectrin antibody orb1528757 at 0.04 µg/ml

Detection of human SPTAN1/Alpha II-Spectrin by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-SPTAN1/Alpha II-Spectrin used at a dilution of 1:200 (1µg/ml).

IHC: Sample: human breast carcinoma. Antibody: Rabbit anti-SPTAN1/Alpha II-Spectrin at 1:200. Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated.