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Catalog Number | orb2291596 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a full length recombinant SPRY2. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 1E10 |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG1 Kappa |
Immunogen | SPRY2 (AAH15745.1, 1 a.a. ~ 315 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | MEARAQSGNGSQPLLQTPRDGGRQRGEPDPRDALTQQVHVLSLDQIRAIRNTNEYTEGPTVVPRPGLKPAPRPSTQHKHERLHGLPEHRQPPRLQHSQVHSSARAPLSRSISTVSSGSRSSTRTSTSSSSSEQRLLGSSFSSGPVADGIIRVQPKSELKPGELKPLSKEDLGLHAYRCEDCGKCKCKECTYPRPLPSDWICDKQCLCSAQNVIDYGTCVCCVKGLFYHCSNDDEDNCADNPCSCSQSHCCTRWSAMGVMSLFLPCLWCYLPAKGCLKLCQGCYDRVNRPGCRCKNSNTVCCKVPTVPPRNFEKPT |
NCBI | AAH15745.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Detection limit for recombinant GST tagged SPRY2 is approximately 0.1 ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to SPRY2 on HeLa cell. [antibody concentration 25 ug/ml]
Immunoperoxidase of monoclonal antibody to SPRY2 on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3 ug/ml]
SPRY2 monoclonal antibody (M01), clone 1E10 Western Blot analysis of SPRY2 expression in C32.
Western Blot analysis of SPRY2 expression in transfected 293T cell line by SPRY2 monoclonal antibody (M01), clone 1E10. Lane 1: SPRY2 transfected lysate (34.7 KDa). Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (60.39 KDa).