SPECC1L Antibody

• Catalog Number: orb1269778
• Category: Antibodies
• Description: SPECC1L Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Gallus, Zebrafish
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This SPECC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 705-733 amino acids from the Central region of human SPECC1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 125 kDa
• Target: SPECC1L
• UniProt ID: Q69YQ0
• NCBI: Q69YQ0
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Cytospin-A, Renal carcinoma antigen NY-REN-22, Spe
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis in HL-60 cell line lysates (35 ug/lane).

Western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane).

SPECC1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of SPECC1 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).