SPDEF monoclonal antibody (M01), clone 4A5

• Catalog Number: orb2291293
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a partial recombinant SPDEF.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4A5
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG2b Kappa
• Immunogen: SPDEF (NP_036523, 92 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: QCPVIDSQAPAGSLDLVPGGLTLEEHSLEQVQSMVVGEVLKDIETACKLLNITADPMDWSPSNVQKWLLWTEHQYRLPPMGKAFQELAGKELCAMSEEQFRQRSPLGGD
• NCBI: NP_036523
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged SPDEF is 0.3 ng/ml as a capture antibody.

Western Blot analysis of SPDEF expression in transfected 293T cell line by SPDEF monoclonal antibody (M01), clone 4A5. Lane 1: SPDEF transfected lysate(37.518 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (37.73 KDa).