Spastin Antibody

• Catalog Number: orb639935
• Category: Antibodies
• Description: The AAA protein family members share an ATPase domain and have roles in various cellular processes including intracellular motility, membrane trafficking, proteolysis, protein folding and organelle biogenesis. Spastin, a member of the AAA protein family, is a 616 amino acid protein and is involved in the function or assembly of nuclear protein complexes. The Spastin protein is expressed ubiquitously and localizes to the nucleus and the cytoplasm, where it may also be involved in microtubule dynamics. Mutations in the Spastin gene (SPAST, SPG4) cause the most common form of spastic paraplegia 4, an autosomal dominant form of hereditary spastic paraplegia (HSP). HSPs comprise a group of inherited neurological disorders characterized by spastic lower extremity weakness due to a length-dependent, retrograde axonopathy of corticospinal motor neurons. SPAST-specific mutations account for approximately 40% of all autosomal dominant HSPs.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length human Spastin protein was used as the immunogen for the Spastin antibody.
• UniProt ID: Q9UP0
• Tested applications: IF, WB
• Dilution range: Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: Sp 6C6
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

Western blot testing of human K562 and Raji cell lysate with Spastin antibody (clone Sp 6C6). Expected molecular weight: 60-70 kDa.

SDS-PAGE analysis of purified, BSA-free Spastin antibody (clone Sp 6C6) as confirmation of integrity and purity.