SPARC Antibody

• Catalog Number: orb19223
• Category: Antibodies
• Description: SPARC Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human SPARC recombinant protein (Position: E107-I303). Human SPARC shares 97% amino acid (aa) sequence identity with both mouse and rat SPARC.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 34632 MW
• UniProt ID: P09486
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: SPARC;Basement-membrane protein 40;BM-40;Osteonect
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis using anti-SPARC antibody.Lane 1:human HUVEC cell;2:human placenta tissue;3:human A375 cell;4:human U20S cell;5:human U87 cell;6:human SH-5Y5Y cell;7:rat testis tissue;8:rat kidney tissue;9:mouse testis tissue;10:mouse kidney tissue.