SPARC Antibody

• Catalog Number: orb1263680
• Category: Antibodies
• Description: SPARC Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus, Mouse, Porcine, Rabbit, Rat, Xenopus
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This SPARC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 35 kDa
• Target: SPARC
• UniProt ID: P09486
• NCBI: P09486
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: SPARC, Basement-membrane protein 40, BM-40, Osteon
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SPARC Antibody in Y79 cell line lysates (35 ug/lane)

Western blot analysis of SPARC using rabbit polyclonal SPARC Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPARC gene.

Formalin-fixed and paraffin-embedded human skin tissue reacted with SPARC Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of widr cells using SPARC Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.