SPAK Antibody

• Catalog Number: orb1526573
• Category: Antibodies
• Description: SPAK Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Immunogen: between 375 and 425
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SPAK
• UniProt ID: Q9UEW8
• NCBI: AAC72238.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: serine/threonine-protein kinase 39; ste-20-related
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human SPAK by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells. Antibodies: Affinity purified rabbit anti-SPAK antibody orb1526573 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human SPAK by immunohistochemistry. Samples: FFPE sections of human stomach cancer and ovarian carcinoma. Antibody: Affinity purified rabbit anti-SPAK (Cat. No. orb1526573) used at a dilution of 1: 200 (1µg/ml). Detection: DAB