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Catalog Number | orb546379 |
---|---|
Category | Antibodies |
Description | SOX15 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human SOX15 recombinant protein (Position: M1-L233). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 29 kDa |
UniProt ID | O60248 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Protein SOX-15; Protein SOX-12; Protein SOX-20; SO Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of SOX15 using anti-SOX15 antibody.Lane 1:human A431 cell;2:human U-87MG cell;3:human HEK293 cell;4:human K562 cell;5:human U2OS cell;6:rat thymus tissue;7:mouse SP20 cell.
IF analysis of SOX15 using anti-SOX15 antibody.SOX15 was detected in immunocytochemical section of A431 cell.
IHC analysis of SOX15 using anti-SOX15 antibody.SOX15 was detected in paraffin-embedded section of mouse brain tissues.
IHC analysis of SOX15 using anti-SOX15 antibody.SOX15 was detected in paraffin-embedded section of rat testis tissues.
IHC analysis of SOX15 using anti-SOX15 antibody.SOX15 was detected in paraffin-embedded section of human testis cancer tissues.
IHC analysis of SOX15 using anti-SOX15 antibody.SOX15 was detected in paraffin-embedded section of mouse testis tissues.
IHC-P, WB | |
Animal, Bovine, Canine, Guinea pig, Human, Mouse, Rat | |
Canine, Equine, Guinea pig, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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