SOS2 Antibody

• Catalog Number: orb1527599
• Category: Antibodies
• Description: SOS2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 1282 and 1332
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SOS2
• UniProt ID: Q07890
• NCBI: NP_008870.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: guanine nucleotide releasing factor; NS9; son of s
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse SOS2 by western blot. Samples: Whole cell lysate (25 µg) from CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SOS2 antibody (orb1527599) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human SOS2 by western blot. Samples: Whole cell lysate (25 µg) from Jurkat, RKO, CAL-27, PANC-1, and MDA-MB-231 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SOS2 antibody (orb1527599) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human SOS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SOS2 antibody (orb1527599) used for IP at 6 µg per reaction. SOS2 was also immunoprecipitated by a previous lot of this antibody (orb1527599) and a second antibody against a different epitope of SOS2.