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Catalog Number | orb215905 |
---|---|
Category | Antibodies |
Description | Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human SLC9A1 recombinant protein (Position: H543-Q815). Human SLC9A1 shares 92% and 93% amino acid (aa) sequences identity with mouse and rat SLC9A1, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Rat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 90763 MW |
UniProt ID | P19634 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Sodium/hydrogen exchanger 1;APNH;Na (+)/H (+) anti Read more... |
Note | For research use only |
Application notes | WB: The detection limit for SLC9A1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of SLC9A1 using anti-SLC9A1 antibody.Lane 1:recombinant human Sodium protein 0.5 ng.
WB analysis of SLC9A1 using anti-SLC9A1 antibody.Lane 1:rat PC-12 cell; 2:human MCF-7 cell.
IHC analysis of SLC9A1 using anti-SLC9A1 antibody. SLC9A1 was detected in a paraffin-embedded section of rat kidney tissue.
IHC analysis of SLC9A1 using anti-SLC9A1 antibody. SLC9A1 was detected in a paraffin-embedded section of human mammary cancer tissue.
IHC analysis of SLC9A1 using anti-SLC9A1 antibody. SLC9A1 was detected in a paraffin-embedded section of mouse kidney tissue.
IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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