SOCS1 antibody

• Catalog Number: orb1427740
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SOCS1.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB17347
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: IF: 1:10~50, WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 23551
• Target: SOCS1
• UniProt ID: O15524
• NCBI: NP_003736.1
• Alternative names: Suppressor of cytokine signaling 1, SOCS-1, JAK-bi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Confocal immunofluorescent analysis of SOCS1 Antibody (N-term) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Western blot:All lanes:Anti-SOCS1 Antibody (N-term) at 1:2000 dilution.Lane 1:mouse thymus lysates;Lane 2:rat spleen lysates.

Western blot analysis of SOCS1 Antibody (N-term) in mouse kidney tissue lysates.SOCS1 (arrow) was detected using the purified Pab.

Immunohistochemical analysis of human colon carcinoma reacted with SOCS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of WiDr cells (right histogram) using SOCS1 Antibody (N-term), compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.