SNAT1 Antibody

• Catalog Number: orb67489
• Category: Antibodies
• Description: Mouse Anti-Rat SNAT1 Monoclonal IgG1
• Target: SLC38A1
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Purification: Protein G Purified
• Immunogen: Fusion protein amino acids 1- 63 of rat SNAT1
• UniProt ID: Q9JM15
• MW: 50kDa
• Tested applications: FC, ICC, IF, IHC, WB
• Dilution range: WB (1:1000)
• Application notes: 1 µg/ml of SMC-401 was sufficient for detection of SNAT1 in 20 µg of lysates from neocortical neurons cultured under amino acid starvation conditions and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Clone Number: N104/32 (Formerly sold as S104-32)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SLC38A1 Antibody, amino acids transporter A1 Antib
• Research Area: Neuroscience, Pharmacology & Drug Discovery, Prote
• Note: For research use only
• Entrez: 170567
• NCBI: NP_620187.1
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SNAT1 Monoclonal Antibody, Clone N104/32. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SNAT1 Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SNAT1 Antibody (D) Composite.

Western Blot analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse Anti-SLC38A1 Monoclonal Antibody, Clone N104/32. Primary Antibody: Mouse Anti-SLC38A1 Monoclonal Antibody at 1:1000.