SLC38A1 Antibody

SKU: orb67489

Description

Mouse Anti-Rat SNAT1 Monoclonal IgG1

Research Area

Neuroscience, Pharmacology & Drug Discovery, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml was sufficient for detection of SNAT1 in 20 µg of lysates from neocortical neurons cultured under amino acid starvation conditions and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N104/32 (Formerly sold as S104-32)
• Immunogen: Fusion protein amino acids 1- 63 of rat SNAT1
• Target: SLC38A1
• Molecular Weight: 50kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SNAT1, SLC38A1, amino acids transporter A1, ATA1, NAT2, sodium coupled neutral amino acids transporter 1, Amino acid transporter system A1, N-system amino acid transporter 2, S38A1, SAT1, SNAT 1, Solute carrier family 38 member 1, System A amino acid transporter 1, System N amino acid transporter 1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SNAT1 Monoclonal Antibody, Clone N104/32. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SNAT1 Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SNAT1 Antibody (D) Composite.

Western Blot analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse Anti-SLC38A1 Monoclonal Antibody, Clone N104/32. Primary Antibody: Mouse Anti-SLC38A1 Monoclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

SLC38A1

UniProt

Q9JM15

NCBI Gene ID

170567

RefSeq (Protein):NP_620187.1