SMYD3 Rabbit Polyclonal Antibody

• Catalog Number: orb581167
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SMYD3
• Target: SMYD3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rat
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Purification: Affinity Purified
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SMYD3
• Protein Sequence: Synthetic peptide located within the following region: PRYPPDSVRLLGRVVFKLMDGAPSESEKLYSFYDLESNINKLTEDKKEGL
• UniProt ID: Q9H7B4
• MW: 42kDa
• Tested applications: ChIP, WB
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KMT3E, ZMYND1, ZNFN3A1, bA74P14.1
• Research Area: Epigenetics & Chromatin
• Note: For research use only
• NCBI: NP_073580

Sample Tissue: Human Ovary Tumor, Antibody dilution: 1 ug/ml.

Positive control (+): HepG2 (HG), Negative control (-): Human stomach (ST), Antibody concentration: 3 ug/ml.

Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

WB Suggested Anti-SMYD3 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: Human Stomach.