SMURF1 Antibody

• Catalog Number: orb334625
• Category: Antibodies
• Description: SMURF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SMURF1 (326-351aa DHNNRTTQFTDPRLHHIMNHQCQLKE), identical to the related mouse sequence.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 86114 MW
• UniProt ID: Q9HCE7
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: E3 ubiquitin-protein ligase SMURF1;hSMURF1;6.3.2.-
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of MCF-7 cells using anti-SMURF1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of SMURF1 using anti-SMURF1 antibody.Lane 1:rat brain tissue;2:MCF-7 cell.

IF analysis of SMURF1 using anti-SMURF1 antibody. SMURF1 was detected in immunocytochemical section of HeLa cells.

IHC analysis of SMURF1 using anti-SMURF1 antibody. SMURF1 was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of SMURF1 using anti-SMURF1 antibody. SMURF1 was detected in paraffin-embedded section of human breast cancer tissue.