SMNDC1 Antibody

• Catalog Number: orb2636871
• Category: Antibodies
• Description: SPF30 (survival of motor neuron-related-splicing factor 30), also known as SMNDC1 (survival motor neuron domain containing 1) or SMNR (SMN-related protein), is an essential splicing factor required for spliceosome assembly that belongs to the SMN family. It contains one Tudor domain with significant similarity to SMN (survival motor neuron) and is expressed in skeletal muscle, pancreas and heart, localizing to Cajal bodies and nuclear speckles. SPF30 plays an important role in spliceosome assembly and directly interacts with five U snRNPs. The loss of SPF30 causes spliceosome assembly to arrest at prespliceosomes (A complex). This supports a function for SPF30 in mediating the incorporation/recruitment of U4/U5/U6 tri-snRNP to the prespliceosome. In addition, the overexpression of SPF30 can lead to apoptosis.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein A/G affinity
• Immunogen: Recombinant full-length human SMNDC1 protein was used as the immunogen for the SMNDC1 antibody.
• UniProt ID: O75940
• Tested applications: FACS, IF
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Application notes: Optimal dilution of the SMNDC1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PCRP-SMNDC1-1A9
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This SMNDC1 antibody is available for research use only.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using SMNDC1 antibody (clone PCRP-SMNDC1-1A9). These results demonstrate the foremost specificity of the PCRP-SMNDC1-1A9 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Immunofluorescent staining of PFA-fixed human HeLa cells using SMNDC1 antibody (green, clone PCRP-SMNDC1-1A9) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with SMNDC1 antibody (blue, clone PCRP-SMNDC1-1A9), and unstained cells (red).

SDS-PAGE analysis of purified, BSA-free SMNDC1 antibody (clone PCRP-SMNDC1-1A9) as confirmation of integrity and purity.