SMC4 Antibody

• Catalog Number: orb614138
• Category: Antibodies
• Description: SMC4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human SMC4 recombinant protein (Position: K911-C1288).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: "Western blot, 0.25-0.5μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human"
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 180 kDa
• UniProt ID: Q9NTJ3
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Structural maintenance of chromosomes protein 4; S
• Note: For research use only
• Application notes: "Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users." . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis using anti-SMC4 antibody.Lane 1:HeLa cell, Lane 2:monkey COS-7 cell, Lane 3:MDA-MB-453 cell, Lane 4:A549 cell, Lane 5:Caco-2 cell.

IF analysis of SMC4 using anti-SMC4 antibody.

Flow Cytometry analysis of SiHa cells using anti-SMC4 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of HL-60 cells using anti-SMC4 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.