You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1274510 |
---|---|
Category | Antibodies |
Description | SMARCE1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human SMARCE1 (NP_003070.3). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 55kDa |
Target | SMARCE1 |
UniProt ID | Q969G3 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | SMARCE1, BAF57 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using SMARCE1 antibody (orb1274510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and SMARCE1 knockout (KO) HeLa cells, using SMARCE1 antibody (orb1274510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Immunofluorescence analysis of HeLa cells using SMARCE1 antibody (orb1274510).
Immunoprecipitation analysis of 150 ug extracts of Jurkat cells using 3 ug SMARCE1 antibody (orb1274510). Western blot was performed from the immunoprecipitate using SMARCE1 antibody (orb1274510) at a dilution of 1:1000.
ELISA, FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, IF, IH, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating