SMARCC1 Antibody

• Catalog Number: orb1274438
• Category: Antibodies
• Description: SMARCC1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-320 of human SMARCC1 (NP_003065.3).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 155kDa
• Target: SMARCC1
• UniProt ID: Q92922
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: SMARCC1, Rsc8, SRG3, SWI3, BAF155, CRACC1
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SMARCC1 antibody (orb1274438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Western blot analysis of extracts from normal (control) and SMARCC1 knockout (KO) HeLa cells, using SMARCC1 antibody (orb1274438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunohistochemistry of paraffin-embedded human kidney cancer using SMARCC1 antibody (orb1274438) at dilution of 1:200 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug SMARCC1 antibody (orb1274438). Western blot was performed from the immunoprecipitate using SMARCC1 antibody (orb1274438) at a dilution of 1:1000.