SMARCA4 Rabbit Polyclonal Antibody

• Catalog Number: orb704395
• Category: Antibodies
• Description: SMARCA4 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SMARCA4 (30-130/1647aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:50-100, IHC-F=1:50-100, ICC/IF=1:100, IF=1:20-50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 181 kDa
• Target: SMARCA4
• UniProt ID: P51532
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: SMCA4_HUMAN; Transcription activator BRG1; EC:3.6.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (KO Validated) SMARCA4 polyclonal Antibody, Unconjugated (orb704395) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SMARCA4) Polyclonal Antibody, Unconjugated (orb704395) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SMARCA4) Polyclonal Antibody, Unconjugated (orb704395) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: 293T (Human) Cell Lysate at 25 ug, Lane 2: SMARCA4 knockout (KO) 293T (Human) Cell Lysate at 25 ug, Primary: Anti-SMARCA4 (orb704395) at 1/500 dilution, Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution, Predicted band size: 180 kD, Observed band size: 180 kD.