SMAD7 Antibody

• Catalog Number: orb1263677
• Category: Antibodies
• Description: SMAD7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This Smad7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-224 amino acids from the Central region of human Smad7.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 46 kDa
• Target: SMAD7
• UniProt ID: O15105
• NCBI: O15105
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Mothers against decapentaplegic homolog 7, MAD hom
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Smad7 Antibody in ZR-75-1 cell line lysates (35 ug/lane)

Smad7 Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Fluorescent confocal image of A431 cell stained with Smad7 Antibody. A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Smad7 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).Smad7 immunoreactivity is localized to cytoplasm significantly.