Smad5 Antibody

• Catalog Number: orb1258375
• Category: Antibodies
• Description: Smad5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 186-465 of human SMAD5 (NP_001001420.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 55kDa
• Target: Smad5
• UniProt ID: Q99717
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: SMAD5, DWFC, JV5-1, MADH5
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SMAD5 antibody (orb1258375) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human lung using Smad5 antibody (orb1258375) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using SMAD5 Polyclonal Antibody (orb1258375) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.