Smad3 (Phospho-Ser423) antibody

• Catalog Number: orb158428
• Category: Antibodies
• Description: Smad3 (Phospho-Ser423) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, WB
• Predicted Reactivity: Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423 (CS(p-S)VS)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ICC=1:100, Flow-Cyt=3μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: phospho-Smad3 (Ser423)
• UniProt ID: P84022
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Smad3 (phospho Ser423); Smad3 (phospho S423); p-Sm
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ICC analysis of Hela cell using phospho-Smad3 (Ser423) polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.

Western blot:Lane 1:Heart (Mouse);Lane 2:Kidney (Mouse);Lane 3:Cerebrum (Rat);Primary:Anti-phospho-Smad3 (Ser423) at 1:1000 dilution.