Smad3 (phospho-Ser204) antibody

• Catalog Number: orb764310
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Smad3 (phospho-Ser204)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser204. AA range:170-219
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 48
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-SMAD3 antibody, anti-MADH3 antibody, anti-Mot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of Hela cell. 1, Smad3 (phospho Ser204) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3A mouse Monoclonal Antibody (5G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of various cells using Phospho-Smad3 (S204) Polyclonal Antibody diluted at 1:500.

Western Blot analysis of MCF-7 cells using Phospho-Smad3 (S204) Polyclonal Antibody diluted at 1:500.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.