SMAD2 (phospho-S255) antibody

• Catalog Number: orb1474578
• Category: Antibodies
• Description: Rabbit monoclonal antibody to SMAD2 (phospho-S255)
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IH, IP, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S255 of human SMAD2 protein. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50)
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Conjugation: Unconjugated
• Target: SMAD2
• Entrez: 4087
• UniProt ID: Q15796
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Alternative names: MADH2; MADR2; Mothers against decapentaplegic homo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SMAD2 (Phospho-S255) expression in K562 (A) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 60 kD)

Immunohistochemical analysis of SMAD2 (Phospho-S255) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.52). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.