Smac/Diablo Antibody

• Catalog Number: orb334593
• Category: Antibodies
• Description: Smac/Diablo Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Smac/Diablo recombinant protein (Position: A56-D239). Human Smac/Diablo shares 88.6% amino acid (aa) sequence identity with mouse Smac/Diablo.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human ELISA , 0.1-0.5μg/ml, Human, -
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 27131 MW
• UniProt ID: Q9NR28
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Diablo homolog, mitochondrial;Direct IAP-binding p
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of K562 cells using anti-Smac/Diablo antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Smac/Diablo using anti-Smac/Diablo antibody.Lane 1:rat testis tissue;2:22RV1 cell;3:SKOV cell.

IF analysis of Smac/Diablo using anti-Smac/Diablo antibody. Smac/Diablo was detected in immunocytochemical section of U20S cells.

IHC analysis of Smac/Diablo using anti-Smac/Diablo antibody. Smac/Diablo was detected in a paraffin-embedded section of mouse testis tissue.

IHC analysis of Smac/Diablo using anti-Smac/Diablo antibody. Smac/Diablo was detected in a paraffin-embedded section of human testis tissue.

IHC analysis of Smac/Diablo using anti-Smac/Diablo antibody. Smac/Diablo was detected in a paraffin-embedded section of rat testis tissue.