You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1927831 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB23028 |
Tested applications | WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:500, WB: 1:1000, WB: 1:1000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 76449 Da |
Target | This SLCO1B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-293 amino acids from the Central region of human SLCO1B1. |
UniProt ID | Q9Y6L6 |
NCBI | NP_006437.3 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Solute carrier organic anion transporter family me Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of SLCO1B1 Antibody (Center) in HepG2 cell line lysates (35 ug/lane). SLCO1B1 (arrow) was detected using the purified Pab.
Western blot analysis of lysate from human liver tissue lysate, using SLCO1B1 Antibody (Center). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
All lanes: Anti-SLCO1B1 Antibody (Center) at 1:500 dilution. Lane 1: Human breast tissue lysate. Lane 2: HepG2 whole cell lysate. Lane 3: SW480 whole cell lysate. Lane 4: NCI-H460 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 76 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.