SLC4A4 antibody

• Catalog Number: orb340892
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SLC4A4
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human NBC1
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: SLC4A4
• Entrez: 54403, 8671, 84484
• UniProt ID: O88343, Q9Y6R1, Q9JI66
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: NBC; NBC1; NBCE1; Electrogenic sodium bicarbonate
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NBC1 expression in PC3 (A), A549 (B) whole cell lysates. (Predicted band size: 72; 112; 116; 121; 123 kD; Observed band size: 135 kD)

Immunofluorescent analysis of NBC1 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).