SLC39A7 Antibody

• Catalog Number: orb1240303
• Category: Antibodies
• Description: SLC39A7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-ZIP7 antibody (orb1240303) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human ZIP7. The immunogen is located within amino acids 20-70 of ZIP7.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-1 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 50kDObserved: 50kD
• Target: SLC39A7
• UniProt ID: Q92504
• NCBI: NP_001070984
• Storage: ZIP7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: ZIP7 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ZIP7 Antibody: KE4, HKE4, ZIP7, RING5, H2-KE4, D6S
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: ZIP7, orb1240303 (1 µg/mL), ZIP7 (4 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: ZIP7, orb1240303 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Brain Tissue and 3T3 (Balb) Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: ZIP7, orb1240303 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Brain Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: ZIP7, orb1240303 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Brain Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: ZIP7, orb1240303 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of ZIP7 in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-ZIP7 antibody (orb1240303) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of ZIP7 in Human Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling ZIP7 with orb1240303 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).