SLC16A11 Antibody

• Catalog Number: orb1270501
• Category: Antibodies
• Description: SLC16A11 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This SLC16A11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-76 amino acids from the N-terminal region of human SLC16A11.
• Concentration: batch dependent
• Dilution range: For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 48 kDa
• Target: SLC16A11
• UniProt ID: Q8NCK7
• NCBI: Q8NCK7
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Monocarboxylate transporter 11, MCT 11, Solute car
• Note: For research use only
• Application notes: For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:500
• Expiration Date: 12 months from date of receipt.

Overlay histogram showing HT-29 cells stained with Antibody (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG1 (1 ug/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

Overlay histogram showing HT-29 cells stained with Antibody (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG1 (1 ug/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

Antibody staining SLC16A11 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:1000 dilution + mouse liver lysates Lysates/proteins at 20 ug per lane.

Western Blot at 1:500 dilution + A549 whole cell lysates Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution + mouse brain lysates Lysates/proteins at 20 ug per lane.

SLC16A11 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.