SLAMF7 Antibody / CS1

• Catalog Number: orb2644417
• Category: Antibodies
• Description: CS1, also known as novel Ly9, SLAMF7, 19A24 or CRACC, is a homophilic cell surface receptor. It is a member of the SLAM (signaling lymphocytic activation molecule) family of receptors expressed on natural killer (NK) cells, T cells and stimulated B cells. CS1 contains immunoreceptor tyrosine-based switch motifs in its cytoplasmic domain but, unlike other SLAM receptors, it does not recruit SAP (SLAM-associated protein). In humans, CS1 activates NK cells through an EAT-2-mediated pathway that is SAP-independent. CS1 recruits and associates with EAT-2, a protein closely related to SAP. EAT-2 induces phosphorylation of CS1 which then, upon ligand binding, activates downstream cytotoxicity effectors PLC and PI 3K. In mice, the EAT-2 association with CS1 has an inhibitory effect on the activation of NK cells.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SLAMF7/3649
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A portion of amino acids 249-335 from the human protein was used as the immunogen for the SLAMF7 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This SLAMF7 antibody is available for research use only.
• UniProt ID: Q9NQ25
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the SLAMF7 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human spleen with SLAMF7 antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil with SLAMF7 antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free SLAMF7 antibody as confirmation of integrity and purity.