SIX2 monoclonal antibody (M01), clone 3D7

• Catalog Number: orb2291506
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant SIX2.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3D7
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: SIX2 (AAH24033, 1 a.a. ~ 291 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MSMLPTFGFTQEQVACVCEVLQQGGNIERLGRFLWSLPACEHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHAKLQQLWLKAHYIEAEKLRGRPLGAVGKYRVRRKFPLPRSIWDGEETSYCFKEKSRSVLREWYAHNPYPSPREKRELTEATGLTTTQVSNWFKNRRQRDRAAEAKERENNENSNSNSHNPLNGSGKSVLGSSEDEKTPSGTPDHSSSSPALLLSPPPPGLPSLHSLGHPPGPSAVPVPVPGGGGADPLQHHHGLQDSILNPMSANLVDLGS
• NCBI: AAH24033
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged SIX2 is 0.03 ng/ml as a capture antibody.

Western Blot analysis of SIX2 expression in transfected 293T cell line by SIX2 monoclonal antibody (M01), clone 3D7. Lane 1: SIX2 transfected lysate(32.3 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of SIX2 over-expressed 293 cell line, cotransfected with SIX2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SIX2 monoclonal antibody (M01), clone 3D7 (Cat # orb2291506). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (57.75 KDa).