SIRT7 Antibody

• Catalog Number: orb251541
• Category: Antibodies
• Description: SIRT7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human SIRT7 recombinant protein (Position: R8-G350). Human SIRT7 shares 96% and 95% amino acid (aa) sequence identity with mouse and rat SIRT7, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 44898 MW
• UniProt ID: Q9NRC8
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: NAD-dependent protein deacetylase sirtuin-7;3.5.1.
• Note: For research use only
• Application notes: WB: The detection limit for SIRT7 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of SIRT7 using anti-SIRT7 antibody.Lane 1:human 293T cell; 2:human HepG2 cell; 3:rat spleen tissue; 4:rat thymus tissue; 5:mouse thyums tissue.

IHC analysis of SIRT7 using anti-SIRT7 antibody. SIRT7 was detected in a paraffin-embedded section of mouse spleen tissue.

IHC analysis of SIRT7 using anti-SIRT7 antibody. SIRT7 was detected in a paraffin-embedded section of rat spleen tissue.