SIRT2 Antibody

• Catalog Number: orb215914
• Category: Antibodies
• Description: SIRT2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SIRT2 (165-187aa YTQNIDTLERIAGLEQEDLVEAH), different from the related mouse and rat sequences by three amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 43182 MW
• UniProt ID: Q8IXJ6
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: NAD-dependent protein deacetylase sirtuin-2;3.5.1.
• Note: For research use only
• Application notes: WB: The detection limit for SIRT2 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of SIRT2 using anti-SIRT2 antibody.Lane 1:recombinant human SIRT2 protein 0.5 ng.

WB analysis of SIRT2 using anti-SIRT2 antibody.Lane 1:rat spleen tissue; 2:human HeLa cell; 3:human A549 cell.

IHC analysis of SIRT2 using anti-SIRT2 antibody. SIRT2 was detected in a paraffin-embedded section of human lung cancer tissue.

IHC analysis of SIRT2 using anti-SIRT2 antibody. SIRT2 was detected in a paraffin-embedded section of rat brain tissue.

IHC analysis of SIRT2 using anti-SIRT2 antibody. SIRT2 was detected in a paraffin-embedded section of mouse brain tissue.