SIRP alpha / CD172a Recombinant Protein

• Catalog Number: orb1231181
• Category: Proteins
• Description: SIRP alpha / CD172a Recombinant Protein
• Tested applications: ELISA, WB
• Tag: Mouse IgG Fc Tag
• Dilution range: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 65.0 kDa. The protein migrates as 90-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Form/Appearance: Lyophilized
• Purity: > 95% as determined by SDS-PAGE.
• MW: 65.0 kDa
• Target: Sirpa
• UniProt ID: P97797
• Protein Sequence: Lys 32 - Asn 373
• Source: HEK293 cells
• NCBI: BAA13520.1, G365
• Storage: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, pH 7.5
• Alternative names: SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1
• Note: For research use only
• Application notes: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 65.0 kDa. The protein migrates as 90-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Mouse SIRP alpha, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Immobilized Mouse SIRP alpha, Mouse IgG2a Fc Tag at 2 ug/ml (100 uL/well) can bind Mouse CD47, Fc Tag with a linear range of 8-63 ng/mL.

Immobilized Human CD47 at 10 ug/ml (100 uL/well) can bind Mouse SIRP alpha, Mouse IgG2a Fc Tag with a linear range of 0.02-0.313 ug/ml.