SIGLEC8 antibody

• Catalog Number: orb20069
• Category: Antibodies
• Description: Goat polyclonal antibody to SIGLEC8
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• MW: 54
• Target: SIGLEC8
• Entrez: 27181
• Protein Sequence: SYKSQLNYKTKQ
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti SIGLEC8 antibody, anti sialic acid binding Ig
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 45kDa band observed in lysates of cell line MOLT4 (calculated MW of 54.0kDa according to NP_055257.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nutku at al, Blood. 2003 Jun 15;101(12):5014-20.; PMID: 12609831). Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20069 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.