SIGLEC10 Antibody (PE)

• Catalog Number: orb1806410
• Category: Antibodies
• Description: SIGLEC10 is a CD33-related receptor of sialoglycans, expressed on eosinophils, monocytes, a subpopulation of NK cells, and at lower level on B cells. Its murine ortholog is Siglec G. SIGLEC10 seems to act as an immunomodulatory receptor, which binds to VAP-1, a glycoprotein expressed on endothelium under inflammatory conditions. Another ligand of SIGLEC10 is CD24, a marker of poorer prognosis in carcinomas.
• Clonality: Monoclonal
• Clone Number: 5G6
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: SIGLEC10 extracellular domain fused with human IgG1 Fc fragment
• Conjugation: PE
• Target: SIGLEC10
• Entrez: 89790
• UniProt ID: Q96LC7
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: PRO940, SGL2
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Expression profiling on peripheral blood subsets using Anti-SIGLEC10 PE antibody (clone 5G6). HCDM CDMaps standardized procedures were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture of anti-human SIGLEC10 PE antibody (clone 5G6, 10 μl reagent / 100 μl of stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, optimized backbone antibody panels (HLDA Innate and HLDA Adaptive) were added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Anti-SIGLEC10 PE antibody (clone 5G6) works in flow cytometry application. Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Mouse monoclonal anti-human SIGLEC10 PE antibody (clone 5G6) was used in amount of 10 µl in 100 µl of blood sample (2 x 10^6 cells).