SIGLEC-8 Antibody

• Catalog Number: orb1246627
• Category: Antibodies
• Description: SIGLEC-8 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SYKSQLNYKTKQ
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SIGLEC8
• UniProt ID: Q9NYZ4
• NCBI: NP_055257.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SIGLEC8, sialic acid binding Ig-like lectin 8, MGC
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 45kDa band observed in lysates of cell line MOLT4 (calculated MW of 54.0kDa according to NP_055257.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nutku at al, Blood. 2003 Jun 15;101(12):5014-20.; PMID: 12609831). Recommended concentration: 0.3-1ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246627 (0.3 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.