SHMT1 Antibody

• Catalog Number: orb1523327
• Category: Antibodies
• Description: SHMT1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 453 and 483
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:500 - 1:2,500; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SHMT1
• UniProt ID: P34896
• NCBI: NP_004160.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: glycine hydroxymethyltransferase; serine methylase
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse SHMT1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody orb1523327 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human SHMT1 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody orb1523327 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human SHMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody orb1523327 used for IP at 6 µg per reaction.

Detection of human SHMT1 by immunohistochemistry.Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-SHMT1 (Cat. No. orb1523327) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of mouse SHMT1 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-SHMT1 (Cat. No. orb1523327) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB