Sheep Human Ig Lambda Light Chains (free) antibody

• Catalog Number: orb22436
• Category: Antibodies
• Description: Precipitating polyclonal sheep antiserum to free human Ig lambda chain. To detect and identify free light chain type and Bence Jones protein in serum or urine by immunoelectrophoresis or double radial immunodiffusion. It is of great importance as an aid in the diagnosis of B cell malignancies such a multiple myeloma and non-Hodgkin lymphoma, and in monitoring their therapy. In immunoelectrophoresis use 1.2 ul of sample in a well with a diameter of 1 mm against 60 ul antiserum in a 20 mm long channel. Electrophoresis for 1 hour, 20 mA. The detection limit of this technique in serum is about 25 mg/l; in the case 100 times concentrated urine is used the detection limit can be brought down to 0.5 mg/l. The fact that in about 40% of the case the Bence Jones protein was only detectable in either serum or urine emphasizes the necessity to investigate both fluids.
• Species/Host: Sheep
• Clonality: Polyclonal
• Form/Appearance: Delipidated, heat inactivated lyophilized whole serum. No preservative added, as it may interfere with the antibody activity.
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Biorbyt accepts no liability for any inaccuracies or omissions in this information.
• UniProt ID: P01834
• Source: A pool of purified Bence Jones lambda proteins isolated from human urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• RRID: AB_10932446
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Delipidated, heat inactivated lyophilized whole serum. No preservative added, as it may interfere with the antibody activity.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.